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KMID : 0545120090190090960
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Journal of Microbiology and Biotechnology
2009 Volume.19 No. 9 p.960 ~ p.965
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Seed-Conjugated Polymer Bead for ￥a2-Microglobulin Removal at Neutral pH
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Kim Mi-Ra
Kang Sung-Soo
Myung Eun-Kyung
Ahn Min-Koo
Choi Jeong-Hyun
Paik Seung-R
Lee Yoon-Sik
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Abstract
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￥a2-Microglobulin (￥a2m) is known to be a major factor for dialysis-related amyloidosis. We have studied ￥a2m removal through an aggregation process, which was initiated by a ligand that could catch the ￥a2m monomer and promote its aggregation into fibril. As a ligand, we have prepared ￥a2m fibril fragments and used them as a seed. The seed was coupled to PEGylated-PS beads to remove the monomeric ￥a2m from solution. The ￥a2m seed-conjugated resin effectively adsorbed the ￥a2m monomers with a capacity of 3.6 mg/ml via promoting their aggregation into fibrils on the resin at pH 7.4.
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KEYWORD
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β2-Microglobulin, dialysis-related amyloidosis, dialysis-related amyloidosis, polymer bead, protein aggregation
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